Bangladesh '18 workshop booklet

06 Sep 2018

Workshop booklet including the following protocols:

  • GN_01: Key characteristics of vector species
  • GN_02: Key characteristics of Culicoides biting midges
  • GN_03: Key characteristics of blackflies
  • GN_04: Key characteristics of Phlebotomine sandflies
  • GN_05: Sticky trapping
  • GN_06: Slide mounting
  • GN_08: Non-destructive DNA extraction from dipteran specimens
  • GN_09: DNA extraction from dipteran specimens, whole blood and cultured cells using Qiagen DNeasy spin columns
  • GN_10: PCR Amplification of the DNA Barcode Segment of the mitochondrial DNA Cytochrome Oxidase Subunit I (COI) gene
  • GN_11: Agarose Gel Electrophoresis using SYBR Safe DNA gel stain
  • GN_12: Agarose Gel Electrophoresis using using Pre-Cast High Throughput SYBR Safe E-Gels
  • GN_13: PCR product purification using MinElute spin columns
  • GN_14: DNA Barcoding introduction to good laboratory practice guide