Protocol: DNA Quality Control Check

06 Jul 2020
Samuel Armoo, Romain Derelle, Daniel Boakye, Mike Y. Osei-Atweneboana, Charles Brockhouse, John Colbourne.

This protocol is for assessing DNA quality and yield prior to sequencing library preparation. This is a very important step given the low affinity of sequencing adapter ligases when it comes to poor DNA quality. Adapted from NanoDrop spectrophotometer (Thermo Scientific), and Qubit fluorometer (ThermoFisher Scientific) manufacturer’s manuals.

nanopore sequencing