Protocol: Non-destructive DNA extraction

06 Sep 2018
Lara L E Harrup

This method details the protocol and guidance notes for the preparation of genomic DNA (gDNA) extractions from Dipteran specimens where a morphological voucher specimen is required. This protocol includes ethanol precipitation to remove PCR inhibitors from the DNA extractions, in addition linear acrylamide or glycogen is included as a co-precipitant to improve DNA yield and facilitate pellet visualisation during precipitation steps. Protocol from the Gnatwork Bangladesh workshop, September 3-6th 2018.