Non-destructive DNA extraction from dipteran specimens
DNA extraction from Dipteran specimens, whole blood and cultured cells using Qiagen DNeasy spin columns
PCR Amplification of the DNA Barcode Segment of the mitochondrial DNA Cytochrome Oxidase Subunit I (COI) gene
Agarose Gel Electrophoresis using SYBR Safe DNA gel stain
Agarose Gel Electrophoresis using Pre-Cast High Throughput SYBR Safe E-Gels
PCR product purification using MinElute spin columns
DNA Barcoding introduction to good laboratory practice guide
Multiplex species-specific PCR-based assays to identify blood meal source
Protocol for preparing and staining sandflies and blackflies prior to micro-computed tomography scanning
This protocol is for the detection of milk casein on small marked arthropods for dispersal and habitat studies.
Nucleic acid extraction and qPCR for detection of parasites from FTA cards
This protocol is for the detection of egg-white ovalbumin on small marked arthropods including Phlebotomine sand flies and Culicoides biting midges for dispersal and habitat studies.
This protocol is for the preparation of Cas9 guide RNA targeted MinION sequencing library from DNA extracted from Simulium sp and Onchocerca volvulus worms. This protocol also describes DNA library loading procedures. This protocol is adapted from Gilpatrick et al. (2020)
This protocol is for assessing DNA quality and yield prior to sequencing library preparation. This is a very important step given the low affinity of sequencing adapter ligases when it comes to poor DNA quality. Adapted from NanoDrop spectrophotometer (Thermo Scientific), and Qubit fluorometer (ThermoFisher Scientific) manufacturer’s manuals.
This protocol is for performing two separate immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans.