Resources

This protocol for field collection and salivary gland dissection of blackflies.

Videos from Principle Investigators and Co-investigators on Gnatwork Projects and on our Management Board with their top tips for Early Career Researchers.

This protocol is for assessing DNA quality and yield prior to sequencing library preparation. This is a very important step given the low affinity of sequencing adapter ligases when it comes to poor DNA quality. Adapted from NanoDrop spectrophotometer (Thermo Scientific), and Qubit fluorometer (ThermoFisher Scientific) manufacturer’s manuals.

This protocol is for the preparation of Cas9 guide RNA targeted MinION sequencing library from DNA extracted from Simulium sp and Onchocerca volvulus worms. This protocol also describes DNA library loading procedures. This protocol is adapted from Gilpatrick et al. (2020)

This protocol is for the detection of egg-white ovalbumin on small marked arthropods including Phlebotomine sand flies and Culicoides biting midges for dispersal and habitat studies.