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Defeat River Blindness
27 Jan 2021
Protocol: Host Decoy Trap Protocol
This protocol is for Host Decoy Traps for the outdoor collection of hematophagous insects.
15 Jan 2021
Protocol: Detection of Onchocerca spp infection in Simulium damnosum s.l. by loop-mediated isothermal amplification (LAMP).
05 Jan 2021
Protocol: Field collection and salivary gland dissection of blackflies
This protocol for field collection and salivary gland dissection of blackflies.
04 Jan 2021
Protocol: Immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans
This protocol is for performing two separate immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans.
21 Sep 2020
Top Tips
Videos from Principle Investigators and Co-investigators on Gnatwork Projects and on our Management Board with their top tips for Early Career Researchers.
06 Jul 2020
Protocol: MinION FASTQ DNA Sequence Analysis
06 Jul 2020
Protocol: DNA Quality Control Check
This protocol is for assessing DNA quality and yield prior to sequencing library preparation. This is a very important step given the low affinity of sequencing adapter ligases when it comes to poor DNA quality. Adapted from NanoDrop spectrophotometer (Thermo Scientific), and Qubit fluorometer (ThermoFisher Scientific) manufacturer’s manuals.
06 Jul 2020
Protocol: DNA Extraction Protocol for Simulium sp. and Onchocerca volvulus
03 Jul 2020
Protocol: Cas9 Targeted Sequencing Protocol for Simulium sp and Onchocerca volvulus
This protocol is for the preparation of Cas9 guide RNA targeted MinION sequencing library from DNA extracted from Simulium sp and Onchocerca volvulus worms. This protocol also describes DNA library loading procedures. This protocol is adapted from Gilpatrick et al. (2020)
08 Jun 2020
Biting Midges World Catelog 2020
Updated catelog produced by Art Borkent and Patrycja Dominiak.
05 Jun 2020
Protocol: Egg-white ovalbumin ELISA protocol
This protocol is for the detection of egg-white ovalbumin on small marked arthropods including Phlebotomine sand flies and Culicoides biting midges for dispersal and habitat studies.
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